› 1kfd

X-ray diffraction
3.9Å resolution

CRYSTAL STRUCTURES OF THE KLENOW FRAGMENT OF DNA POLYMERASE I COMPLEXED WITH DEOXYNUCLEOSIDE TRIPHOSPHATE AND PYROPHOSPHATE

Released:
DOI: 10.2210/pdb1kfd/pdb
PDB entry 1kfd coloured by chain, front view.
PDB entry 1kfd coloured by chain, side view.
PDB entry 1kfd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate.
Beese LS, Friedman JM, Steitz TA
Biochemistry 32 14095-101 (1993)
PMID: 8260491

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 68.16 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P00582 (Residues: 324-928; Coverage: 65%)
Gene names: JW3835, b3863, polA, resA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CTP 1 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43
Unit cell:
a: 104.3Å b: 104.3Å c: 86Å
α: 90° β: 90° γ: 90°
Expression system: Not provided

Quick links

Citations

11 review citations

DNA replication and transcription in mammalian mitochondria.
Falkenberg et al. (2007)
10 more

9 mentions without citation

Building better polymerases: Engineering the replication of expanded genetic alphabets.
Ouaray et al. (2020)
8 more