PDBe 1keq

X-ray diffraction
1.88Å resolution

Crystal Structure of F65A/Y131C Carbonic Anhydrase V, covalently modified with 4-chloromethylimidazole

Released:

Function and Biology Details

Reaction catalysed:
H(2)CO(3) = CO(2) + H(2)O. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 5A, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 28.14 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P23589 (Residues: 53-299; Coverage: 83%)
Gene names: Ca5, Ca5a, Car5, Car5a
Structure domains: Carbonic Anhydrase II

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: C2
Unit cell:
a: 99.299Å b: 66.703Å c: 92.563Å
α: 90° β: 105.7° γ: 90°
R-values:
R R work R free
0.185 0.185 0.217
Expression system: Escherichia coli