PDBe 1kee

X-ray diffraction
2.1Å resolution

Inactivation of the Amidotransferase Activity of Carbamoyl Phosphate Synthetase by the Antibiotic Acivicin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero octamer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carbamoyl-phosphate synthase large chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 1073 amino acids
Theoretical weight: 117.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Best match: P00968 (Residues: 1-1073)
Gene names: JW0031, b0033, carB, pyrA
Carbamoyl-phosphate synthase small chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 382 amino acids
Theoretical weight: 41.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Best match: P0A6F1 (Residues: 1-382)
Gene names: JW0030, b0032, carA, pyrA

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 152.4Å b: 164.4Å c: 333.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.211
Expression system: Escherichia coli