PDBe 1k93

X-ray diffraction
2.95Å resolution

Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 144 amino acids
Theoretical weight: 16.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Best match: P0DP23 (Residues: 6-149)
Gene names: CALM, CALM1, CAM, CAM1
Calmodulin-sensitive adenylate cyclase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 510 amino acids
Theoretical weight: 58.81 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21
UniProt:
  • Best match: P40136 (Residues: 291-800)
Gene names: BXA0141, GBAA_pXO1_0142, cya, pXO1-122

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I222
Unit cell:
a: 116.73Å b: 167.311Å c: 344.296Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.278 0.315
Expression system: Escherichia coli BL21