PDBe 1k3z

X-ray diffraction
2.5Å resolution

X-ray crystal structure of the IkBb/NF-kB p65 homodimer complex

Released:
Source organism: Mus musculus
Primary publication:
X-ray crystal structure of an IkappaBbeta x NF-kappaB p65 homodimer complex.
J. Biol. Chem. 278 23094-100 (2003)
PMID: 12686541

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription factor p65 Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04207 (Residues: 191-326; Coverage: 25%)
Gene names: Nfkb3, Rela
Sequence domains: Rel homology dimerisation domain
Structure domains: Immunoglobulins
NF-kappa-B inhibitor beta Chain: D
Molecule details ›
Chain: D
Length: 282 amino acids
Theoretical weight: 30.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q60778 (Residues: 50-331; Coverage: 79%)
Gene names: Ikbb, Nfkbib
Sequence domains: Ankyrin repeat
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 46.4Å b: 48.92Å c: 59.47Å
α: 95.17° β: 91.8° γ: 105.45°
R-values:
R R work R free
0.181 0.181 0.256
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)