PDBe 1jxh

X-ray diffraction
2.3Å resolution

4-Amino-5-hydroxymethyl-2-methylpyrimidine Phosphate Kinase from Salmonella typhimurium

Released:

Function and Biology Details

Reactions catalysed:
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-2- methyl-5-(phosphomethyl)pyrimidine. 
ATP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = ADP + 4-amino-2- methyl-5-(diphosphooxymethyl)pyrimidine. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 31.27 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55882 (Residues: 1-266; Coverage: 100%)
Gene names: STM2146, thiD
Sequence domains: Phosphomethylpyrimidine kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1, APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 77.724Å b: 77.724Å c: 183.419Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.272
Expression system: Escherichia coli BL21(DE3)