› 1jxa

X-ray diffraction
3.1Å resolution

GLUCOSAMINE 6-PHOSPHATE SYNTHASE WITH GLUCOSE 6-PHOSPHATE

Released:
DOI: 10.2210/pdb1jxa/pdb
PDB entry 1jxa coloured by chain, front view.
PDB entry 1jxa coloured by chain, side view.
PDB entry 1jxa coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Channeling of ammonia in glucosamine-6-phosphate synthase.
Teplyakov A, Obmolova G, Badet B, Badet-Denisot MA
J Mol Biol 313 1093-102 (2001)
PMID: 11700065

Function and Biology Details

Reaction catalysed: 
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 608 amino acids
Theoretical weight: 66.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17169 (Residues: 2-609; Coverage: 100%)
Gene names: JW3707, b3729, glmS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand G6Q 2 x G6Q
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 131.4Å b: 112.4Å c: 185.1Å
α: 90° β: 96.4° γ: 90°
R-values:
R R work R free
0.22 0.2 0.28
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
11 more

9 mentions without citation

A novel N-terminal domain may dictate the glucose response of Mondo proteins.
McFerrin et al. (2012)
8 more