1jws Citations

Structural, energetic, and functional analysis of a protein-protein interface at distinct stages of affinity maturation.

Structure 11 1151-61 (2003)
Related entries: 1jwm, 1jwu

Cited: 15 times
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Due to a paucity of studies that synthesize structural, energetic, and functional analyses of a series of protein complexes representing distinct stages in an affinity maturation pathway, the biophysical basis for the molecular evolution of protein-protein interactions is poorly understood. Here, we combine crystal structures and binding-free energies of a series of variant superantigen (SAG)-major histocompatibility complex (MHC) class II complexes exhibiting increasingly higher affinity to reveal that this affinity maturation pathway is controlled largely by two biophysical factors: shape complementarity and buried hydrophobic surface. These factors, however, do not contribute equivalently to the affinity maturation of the interface, as the former dominates the early steps of the maturation process while the latter is responsible for improved binding in later steps. Functional assays reveal how affinity maturation of the SAG-MHC interface corresponds to T cell activation by SAGs.

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  4. Crystal structure of staphylococcal enterotoxin I (SEI) in complex with a human major histocompatibility complex class II molecule. Fernández MM, Guan R, Swaminathan CP, Malchiodi EL, Mariuzza RA. J. Biol. Chem. 281 25356-25364 (2006)
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  7. Bacterial superantigens promote acute nasopharyngeal infection by Streptococcus pyogenes in a human MHC Class II-dependent manner. Kasper KJ, Zeppa JJ, Wakabayashi AT, Xu SX, Mazzuca DM, Welch I, Baroja ML, Kotb M, Cairns E, Cleary PP, Haeryfar SM, McCormick JK. PLoS Pathog. 10 e1004155 (2014)
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  10. Flanking residues are central to DO11.10 T cell hybridoma stimulation by ovalbumin 323-339. Roy BM, Zhukov DV, Maynard JA. PLoS ONE 7 e47585 (2012)
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  12. Molecular basis of in vitro affinity maturation and functional evolution of a neutralizing anti-human GM-CSF antibody. Eylenstein R, Weinfurtner D, Härtle S, Strohner R, Böttcher J, Augustin M, Ostendorp R, Steidl S. MAbs 8 176-186 (2016)