PDBe 1jw9

X-ray diffraction
1.7Å resolution

Structure of the Native MoeB-MoaD Protein Complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Molybdopterin-synthase adenylyltransferase Chain: B
Molecule details ›
Chain: B
Length: 249 amino acids
Theoretical weight: 26.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P12282 (Residues: 1-249; Coverage: 100%)
Gene names: JW0810, b0826, chlN, moeB
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain
Molybdopterin synthase sulfur carrier subunit Chain: D
Molecule details ›
Chain: D
Length: 81 amino acids
Theoretical weight: 8.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30748 (Residues: 1-81; Coverage: 100%)
Gene names: JW0767, b0784, chlA4, chlM, moaD
Sequence domains: ThiS family
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P41212
Unit cell:
a: 77.832Å b: 77.832Å c: 102.139Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.209
Expression system: Escherichia coli BL21