PDBe 1ju5

Solution NMR

Ternary complex of an Crk SH2 domain, Crk-derived phophopeptide, and Abl SH3 domain by NMR spectroscopy

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tyrosine-protein kinase ABL1 Chain: C
Molecule details ›
Chain: C
Length: 61 amino acids
Theoretical weight: 6.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00519 (Residues: 62-121; Coverage: 5%)
Gene names: ABL, ABL1, JTK7
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Adapter molecule crk Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P46108 (Residues: 12-120; Coverage: 36%)
Gene name: CRK
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein
Adapter molecule crk Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q64010 (Residues: 217-228; Coverage: 4%)
Gene names: Crk, Crko

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Refinement method: ARIA 1.0, CNS 1.0
Expression system: Escherichia coli BL21