PDBe 1jsu

X-ray diffraction
2.3Å resolution

P27(KIP1)/CYCLIN A/CDK2 COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-dependent kinase inhibitor 1B Chain: C
Molecule details ›
Chain: C
Length: 84 amino acids
Theoretical weight: 9.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46527 (Residues: 23-106; Coverage: 42%)
Gene names: CDKN1B, KIP1
Sequence domains: Cyclin-dependent kinase inhibitor
Structure domains: p27
Cyclin-A2 Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 29.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20248 (Residues: 173-432; Coverage: 60%)
Gene names: CCN1, CCNA, CCNA2
Sequence domains:
Structure domains: Cyclin A; domain 1
Cyclin-dependent kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 34.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24941 (Residues: 1-298; Coverage: 100%)
Gene names: CDK2, CDKN2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 73.8Å b: 78.3Å c: 137.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.26
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda