› 1jqa

X-ray diffraction
2.05Å resolution

Bacillus stearothermophilus glycerol dehydrogenase complex with glycerol

Released:
DOI: 10.2210/pdb1jqa/pdb
PDB entry 1jqa coloured by chain, front view.
PDB entry 1jqa coloured by chain, side view.
PDB entry 1jqa coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Glycerol dehydrogenase. structure, specificity, and mechanism of a family III polyol dehydrogenase.
Ruzheinikov SN, Burke J, Sedelnikova S, Baker PJ, Taylor R, Bullough PA, Muir NM, Gore MG, Rice DW
Structure 9 789-802 (2001)
PMID: 11566129

Function and Biology Details

Reaction catalysed: 
Glycerol + NAD(+) = glycerone + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo octamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 39.56 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32816 (Residues: 1-370; Coverage: 100%)
Gene names: gld, gldA
Sequence domains: Iron-containing alcohol dehydrogenase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: I422
Unit cell:
a: 105.91Å b: 105.91Å c: 149.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.202
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Key enzymes catalyzing glycerol to 1,3-propanediol.
Jiang et al. (2016)
1 more

8 mentions without citation

A Hitchhiker's Guide to Supplying Enzymatic Reducing Power into Synthetic Cells.
Partipilo et al. (2023)
7 more