› 1jpj

X-ray diffraction
2.3Å resolution

GMPPNP Complex of SRP GTPase NG Domain

Released:
DOI: 10.2210/pdb1jpj/pdb
PDB entry 1jpj coloured by chain, front view.
PDB entry 1jpj coloured by chain, side view.
PDB entry 1jpj coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
The conformation of bound GMPPNP suggests a mechanism for gating the active site of the SRP GTPase.
Padmanabhan S, Freymann DM
Structure 9 859-67 (2001)
PMID: 11566135

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 32.47 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O07347 (Residues: 1-296; Coverage: 69%)
Gene name: ffh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 52.69Å b: 60.41Å c: 86.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.306
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
5 more

7 mentions without citation

Molecular mechanism of co-translational protein targeting by the signal recognition particle.
Saraogi et al. (2011)
6 more