PDBe 1jof

X-ray diffraction
2.5Å resolution

Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme

Released:

Function and Biology Details

Reaction catalysed:
3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxy-cis,cis-muconate cyclase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 365 amino acids
Theoretical weight: 41.72 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli
UniProt:
  • Canonical: P38677 (Residues: 2-366; Coverage: 100%)
Gene name: NCU04071
Sequence domains: Lactonase, 7-bladed beta-propeller
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14, null
Spacegroup: P212121
Unit cell:
a: 92.29Å b: 160.6Å c: 237.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.251
Expression system: Escherichia coli