PDBe 1jk1

X-ray diffraction
1.9Å resolution

Zif268 D20A Mutant Bound to WT DNA Site

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Early growth response protein 1 Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.81 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08046 (Residues: 333-421; Coverage: 17%)
Gene names: Egr-1, Egr1, Krox-24
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5'-D(*AP*GP*CP*GP*TP*GP*GP*GP*CP*GP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.46 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*CP*CP*GP*CP*CP*CP*AP*CP*GP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.26 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 44.26Å b: 56.24Å c: 131.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.266
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided