› 1jht

X-ray diffraction
2.15Å resolution

Crystal structure of HLA-A2*0201 in complex with a nonameric altered peptide ligand (ALGIGILTV) from the MART-1/Melan-A.

Released:
DOI: 10.2210/pdb1jht/pdb
PDB entry 1jht coloured by chain, front view.
PDB entry 1jht coloured by chain, side view.
PDB entry 1jht coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of two closely related but antigenically distinct HLA-A2/melanocyte-melanoma tumor-antigen peptide complexes.
Sliz P, Michielin O, Cerottini JC, Luescher I, Romero P, Karplus M, Wiley DC
J Immunol 167 3276-84 (2001)
PMID: 11544315

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137723 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
nonameric peptide ligand from the MART-1/Melan-A Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 856 Da

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 49.19Å b: 74.47Å c: 122.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.252
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Antigenicity and immunogenicity of Melan-A/MART-1 derived peptides as targets for tumor reactive CTL in human melanoma.
Romero et al. (2002)
5 more

8 mentions without citation

Modeling the structure of bound peptide ligands to major histocompatibility complex.
Tong et al. (2004)
7 more