PDBe 1j7u

X-ray diffraction
2.4Å resolution

Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type IIIa AMPPNP Complex

Source organism: Enterococcus faecalis

Function and Biology Details

Reaction catalysed:
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Aminoglycoside 3'-phosphotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 31.01 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P0A3Y5 (Residues: 1-264; Coverage: 100%)
Gene name: aphA
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 50.168Å b: 91.321Å c: 132.653Å
α: 90° β: 90° γ: 90°
R R work R free
0.217 0.217 0.277
Expression system: Escherichia coli BL21(DE3)