PDBe 1j79

X-ray diffraction
1.7Å resolution

Molecular Structure of Dihydroorotase: A Paradigm for Catalysis Through the Use of a Binuclear Metal Center


Function and Biology Details

Reaction catalysed:
(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydroorotase Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 38.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P05020 (Residues: 2-348; Coverage: 100%)
Gene names: JW1049, b1062, pyrC
Sequence domains: Amidohydrolase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 51.6Å b: 78.8Å c: 180.3Å
α: 90° β: 90° γ: 90°
R R work R free
0.193 0.191 0.258
Expression system: Escherichia coli