› 1j5n

Solution NMR

Solution Structure of the Non-Sequence-Specific HMGB protein NHP6A in complex with SRY DNA

Released:
DOI: 10.2210/pdb1j5n/pdb
PDB entry 1j5n coloured by chain, ensemble of 20 models, front view.
PDB entry 1j5n coloured by chain, ensemble of 20 models, side view.
PDB entry 1j5n coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The S. cerevisiae architectural HMGB protein NHP6A complexed with DNA: DNA and protein conformational changes upon binding.
Masse JE, Wong B, Yen YM, Allain FH, Johnson RC, Feigon J
J Mol Biol 323 263-84 (2002)
PMID: 12381320

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113990 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Non-histone chromosomal protein 6A Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.82 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11632 (Residues: 1-93; Coverage: 100%)
Gene names: NHP6A, NHPA, YP9499.09C, YPR052C
Sequence domains: HMG (high mobility group) box
Structure domains: High mobility group box domain
5'-D(*GP*GP*GP*GP*TP*GP*AP*TP*TP*GP*TP*TP*CP*AP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*CP*TP*GP*AP*AP*CP*AP*AP*TP*CP*AP*CP*CP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

HMGB proteins and gene expression.
Agresti et al. (2003)
10 more

28 mentions without citation

The histone chaperone FACT: structural insights and mechanisms for nucleosome reorganization.
Winkler et al. (2011)
27 more