› 1j56

Solution NMR

MINIMIZED AVERAGE STRUCTURE OF BERYLLOFLUORIDE-ACTIVATED NTRC RECEIVER DOMAIN: MODEL STRUCTURE INCORPORATING ACTIVE SITE CONTACTS

Released:
DOI: 10.2210/pdb1j56/pdb
PDB entry 1j56 coloured by chain, front view.
PDB entry 1j56 coloured by chain, side view.
PDB entry 1j56 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
High-resolution solution structure of the beryllofluoride-activated NtrC receiver domain.
Hastings CA, Lee SY, Cho HS, Yan D, Kustu S, Wemmer DE
Biochemistry 42 9081-90 (2003)
PMID: 12885241

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional regulator NtrC Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.64 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P41789 (Residues: 1-124; Coverage: 26%)
Gene names: STM4005, glnG, ntrC
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand BEF 1 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, torsion angle dynamics, simulated annealing, ENERGY MINIMIZATION
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
5 more

1 mention without citation

Self-guided Langevin dynamics study of regulatory interactions in NtrC.
Damjanović et al. (2009)