PDBe 1j25

X-ray diffraction
1.78Å resolution

Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain, Mn cocrystal

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase, putative Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.12 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZH8 (Residues: 548-689; Coverage: 19%)
Gene name: PF2015
Sequence domains: ERCC4 domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3121
Unit cell:
a: 69.795Å b: 69.795Å c: 58.509Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.252
Expression system: Escherichia coli