› 1j23

X-ray diffraction
1.78Å resolution

Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain

Released:
DOI: 10.2210/pdb1j23/pdb
PDB entry 1j23 coloured by chain, front view.
PDB entry 1j23 coloured by chain, side view.
PDB entry 1j23 coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
X-ray and biochemical anatomy of an archaeal XPF/Rad1/Mus81 family nuclease: similarity between its endonuclease domain and restriction enzymes.
Nishino T, Komori K, Ishino Y, Morikawa K
Structure 11 445-57 (2003)
PMID: 12679022

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase, putative Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.12 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZH8 (Residues: 548-689; Coverage: 19%)
Gene name: PF2015
Sequence domains: ERCC4 domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3121
Unit cell:
a: 69.795Å b: 69.795Å c: 58.509Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.248
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
17 more

5 mentions without citation

DNA Repair Repertoire of the Enigmatic Hydra.
Barve et al. (2021)
4 more