PDBe 1j1e

X-ray diffraction
3.3Å resolution

Crystal structure of the 52kDa domain of human cardiac troponin in the Ca2+ saturated form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Troponin C, slow skeletal and cardiac muscles Chains: A, D
Molecule details ›
Chains: A, D
Length: 161 amino acids
Theoretical weight: 18.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 1-161; Coverage: 100%)
Gene names: TNNC, TNNC1
Sequence domains:
Structure domains: EF-hand
Troponin I, cardiac muscle Chains: C, F
Molecule details ›
Chains: C, F
Length: 180 amino acids
Theoretical weight: 20.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19429 (Residues: 31-210; Coverage: 86%)
Gene names: TNNC1, TNNI3
Sequence domains: Troponin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Troponin T, cardiac muscle Chains: B, E
Molecule details ›
Chains: B, E
Length: 106 amino acids
Theoretical weight: 12.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45379 (Residues: 193-298; Coverage: 36%)
Gene name: TNNT2
Sequence domains: Troponin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 48.299Å b: 169.506Å c: 68.538Å
α: 90° β: 102.38° γ: 90°
R-values:
R R work R free
0.251 0.251 0.308
Expression system: Escherichia coli