PDBe 1j1d

X-ray diffraction
2.61Å resolution

Crystal structure of the 46kDa domain of human cardiac troponin in the Ca2+ saturated form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Troponin C, slow skeletal and cardiac muscles Chains: A, D
Molecule details ›
Chains: A, D
Length: 161 amino acids
Theoretical weight: 18.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 1-161; Coverage: 100%)
Gene names: TNNC, TNNC1
Sequence domains:
Structure domains: EF-hand
Troponin I, cardiac muscle Chains: C, F
Molecule details ›
Chains: C, F
Length: 133 amino acids
Theoretical weight: 15.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19429 (Residues: 31-163; Coverage: 63%)
Gene names: TNNC1, TNNI3
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Troponin T, cardiac muscle Chains: B, E
Molecule details ›
Chains: B, E
Length: 106 amino acids
Theoretical weight: 12.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45379 (Residues: 193-298; Coverage: 36%)
Gene name: TNNT2
Sequence domains: Troponin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 42.346Å b: 167.86Å c: 69.707Å
α: 90° β: 101.35° γ: 90°
R-values:
R R work R free
0.264 0.264 0.298
Expression system: Escherichia coli