PDBe 1j00

X-ray diffraction
2Å resolution

E. coli Thioesterase I/Protease I/Lysophospholipase L1 in complexed with diethyl phosphono moiety

Released:

Function and Biology Details

Reactions catalysed:
Palmitoyl-CoA + H(2)O = CoA + palmitate. 
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate. 
2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate. 
A phenyl acetate + H(2)O = a phenol + acetate. 
Biochemical function:
Biological process:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioesterase 1/protease 1/lysophospholipase L1 Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADA1 (Residues: 27-208; Coverage: 100%)
Gene names: JW0483, apeA, b0494, pldC, tesA
Sequence domains: GDSL-like Lipase/Acylhydrolase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P43212
Unit cell:
a: 49.907Å b: 49.907Å c: 171.898Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.267
Expression system: Escherichia coli BL21(DE3)