PDBe 1iwq

X-ray diffraction
2Å resolution

Crystal Structure of MARCKS calmodulin binding domain peptide complexed with Ca2+/Calmodulin

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myristoylated alanine-rich C-kinase substrate Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.33 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P26645 (Residues: 148-166; Coverage: 6%)
Gene names: Macs, Marcks
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP23 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX
Spacegroup: P6122
Unit cell:
a: 40.229Å b: 40.229Å c: 343.468Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.223 0.267
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)