› 1ion

X-ray diffraction
2.3Å resolution

THE SEPTUM SITE-DETERMINING PROTEIN MIND COMPLEXED WITH MG-ADP FROM PYROCOCCUS HORIKOSHII OT3

Released:
DOI: 10.2210/pdb1ion/pdb
PDB entry 1ion coloured by chain, front view.
PDB entry 1ion coloured by chain, side view.
PDB entry 1ion coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
The three-dimensional structure of septum site-determining protein MinD from Pyrococcus horikoshii OT3 in complex with Mg-ADP.
Sakai N, Yao M, Itou H, Watanabe N, Yumoto F, Tanokura M, Tanaka I
Structure 9 817-26 (2001)
PMID: 11566131

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AAA domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 26.5 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58346 (Residues: 1-243; Coverage: 99%)
Gene name: PH0612
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P213
Unit cell:
a: 96.59Å b: 96.59Å c: 96.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.188
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Assembly dynamics of the bacterial MinCDE system and spatial regulation of the Z ring.
Lutkenhaus J. (2007)
8 more

3 mentions without citation

Bacterial chromosome segregation: structure and DNA binding of the Soj dimer--a conserved biological switch.
Leonard et al. (2005)
2 more