PDBe 1ik9

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX

Released:
Source organism: Homo sapiens
Primary publication:
Crystal structure of an Xrcc4-DNA ligase IV complex.
Nat. Struct. Biol. 8 1015-9 (2001)
PMID: 11702069

Function and Biology Details

Reaction catalysed:
ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA ligase 4 Chain: C
Molecule details ›
Chain: C
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49917 (Residues: 748-784; Coverage: 4%)
Gene name: LIG4
Sequence domains: DNA ligase IV
DNA repair protein XRCC4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 213 amino acids
Theoretical weight: 24.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13426 (Residues: 1-213; Coverage: 63%)
Gene name: XRCC4
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 45.062Å b: 79.628Å c: 242.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.266
Expression system: Escherichia coli BL21(DE3)