PDBe 1i8d

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF RIBOFLAVIN SYNTHASE

Released:
Source organism: Escherichia coli
Primary publication:
Crystal structure of riboflavin synthase.
Structure 9 399-408 (2001)
PMID: 11377200

Function and Biology Details

Reaction catalysed:
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D- ribitylamino)-5-amino-2,6-dihydroxypyrimidine. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 213 amino acids
Theoretical weight: 23.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFU8 (Residues: 1-213; Coverage: 100%)
Gene names: JW1654, b1662, ribC, ribE
Sequence domains: Lumazine binding domain
Structure domains: Elongation Factor Tu (Ef-tu); domain 3

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 52.083Å b: 61.705Å c: 219.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.299
Expression system: Escherichia coli BL21(DE3)