PDBe 1i6o

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF E. COLI BETA CARBONIC ANHYDRASE (ECCA)

Released:

Function and Biology Details

Reaction catalysed:
H(2)CO(3) = CO(2) + H(2)O. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 25.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61517 (Residues: 1-220; Coverage: 100%)
Gene names: JW0122, b0126, can, cynT2, yadF
Sequence domains: Carbonic anhydrase
Structure domains: Beta-carbonic Anhydrase; Chain A

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P4322
Unit cell:
a: 81.244Å b: 81.244Å c: 162.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.243
Expression system: Escherichia coli BL21