PDBe 1i6h

X-ray diffraction
3.3Å resolution

RNA POLYMERASE II ELONGATION COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
10 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (12 distinct):
DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT Chain: A
DNA-DIRECTED RNA POLYMERASE II 45KD POLYPEPTIDE Chain: C
Molecule details ›
Chain: C
Length: 318 amino acids
Theoretical weight: 35.33 KDa
Source organism: Saccharomyces cerevisiae
Structure domains:
DNA-DIRECTED RNA POLYMERASE II 27KD POLYPEPTIDE Chain: E
Molecule details ›
Chain: E
Length: 215 amino acids
Theoretical weight: 25.12 KDa
Source organism: Saccharomyces cerevisiae
Structure domains:
DNA-DIRECTED RNA POLYMERASE II 23KD POLYPEPTIDE Chain: F
Molecule details ›
Chain: F
Length: 155 amino acids
Theoretical weight: 17.93 KDa
Source organism: Saccharomyces cerevisiae
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
DNA-DIRECTED RNA POLYMERASE II 14.5KD POLYPEPTIDE Chain: H
Molecule details ›
Chain: H
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae
Structure domains: Nucleic acid-binding proteins
DNA-DIRECTED RNA POLYMERASE II 14.2KD POLYPEPTIDE Chain: I
Molecule details ›
Chain: I
Length: 122 amino acids
Theoretical weight: 14.31 KDa
Source organism: Saccharomyces cerevisiae
Structure domains: N-terminal domain of TfIIb
DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE Chain: J
Molecule details ›
Chain: J
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae
Structure domains: Homeodomain-like
DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE Chain: K
Molecule details ›
Chain: K
Length: 120 amino acids
Theoretical weight: 13.63 KDa
Source organism: Saccharomyces cerevisiae
Structure domains: RNA polymerase, RBP11-like subunit
DNA-DIRECTED RNA POLYMERASE II 7.7KD POLYPEPTIDE Chain: L
Molecule details ›
Chain: L
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Saccharomyces cerevisiae
Structure domains: RNA polymerase ii, chain L
5'-D(P*AP*AP*AP*TP*GP*CP*CP*TP*GP*GP*TP*CP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
5'-R(P*GP*AP*CP*CP*AP*GP*GP*CP*A)-3' Chain: R
Molecule details ›
Chain: R
Length: 9 nucleotides
Theoretical weight: 2.89 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 157.3Å b: 220.7Å c: 191.3Å
α: 90° β: 97.5° γ: 90°
R-values:
R R work R free
0.25 0.25 0.298