› 1hxh

X-ray diffraction
1.22Å resolution

COMAMONAS TESTOSTERONI 3BETA/17BETA HYDROXYSTEROID DEHYDROGENASE

Released:
DOI: 10.2210/pdb1hxh/pdb
PDB entry 1hxh coloured by chain, front view.
PDB entry 1hxh coloured by chain, side view.
PDB entry 1hxh coloured by chain, top view.
Source organism: Comamonas testosteroni
Primary publication:
Structure of bacterial 3beta/17beta-hydroxysteroid dehydrogenase at 1.2 A resolution: a model for multiple steroid recognition.
Benach J, Filling C, Oppermann UC, Roversi P, Bricogne G, Berndt KD, Jörnvall H, Ladenstein R
Biochemistry 41 14659-68 (2002)
PMID: 12475215

Function and Biology Details

Reaction catalysed: 
Testosterone + NAD(P)(+) = androst-4-ene-3,17-dione + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-beta-hydroxysteroid dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 253 amino acids
Theoretical weight: 26.85 KDa
Source organism: Comamonas testosteroni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19871 (Residues: 2-254; Coverage: 100%)
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 80.024Å b: 110.595Å c: 115.143Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.146 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Dual substrate recognition of aminotransferases.
Hirotsu et al. (2005)
26 other articles

9 mentions without citation

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
8 more