PDBe 1hto

X-ray diffraction
2.4Å resolution

CRYSTALLOGRAPHIC STRUCTURE OF A RELAXED GLUTAMINE SYNTHETASE FROM MYCOBACTERIUM TUBERCULOSIS

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo 12-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine synthetase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 477 amino acids
Theoretical weight: 53.5 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WN39 (Residues: 2-478; Coverage: 100%)
Gene names: MTCY190.31, MTCY427.01, Rv2220, glnA, glnA1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P212121
Unit cell:
a: 207.72Å b: 257.69Å c: 274.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.255
Expression system: Escherichia coli