PDBe 1hr6

X-ray diffraction
2.5Å resolution

Yeast Mitochondrial Processing Peptidase

Released:

Function and Biology Details

Reaction catalysed:
Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2. 
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial-processing peptidase subunit beta Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 443 amino acids
Theoretical weight: 48.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10507 (Residues: 21-462; Coverage: 96%)
Gene names: L9632.10, MAS1, MIF1, YLR163C
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain A, domain 1
Mitochondrial-processing peptidase subunit alpha Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 475 amino acids
Theoretical weight: 52.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11914 (Residues: 14-482; Coverage: 97%)
Gene names: MAS2, MIF2, YHR024C
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain A, domain 1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 134.18Å b: 178.5Å c: 202.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.279
Expression system: Escherichia coli BL21(DE3)