› 1ho8

X-ray diffraction
2.95Å resolution

CRYSTAL STRUCTURE OF THE REGULATORY SUBUNIT H OF THE V-TYPE ATPASE OF SACCHAROMYCES CEREVISIAE

Released:
DOI: 10.2210/pdb1ho8/pdb
PDB entry 1ho8 coloured by chain, front view.
PDB entry 1ho8 coloured by chain, side view.
PDB entry 1ho8 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the regulatory subunit H of the V-type ATPase of Saccharomyces cerevisiae.
Sagermann M, Stevens TH, Matthews BW
Proc Natl Acad Sci U S A 98 7134-9 (2001)
PMID: 11416198

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
V-type proton ATPase subunit H Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 54.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41807 (Residues: 1-478; Coverage: 100%)
Gene names: CLS11, VMA13, YP3085.02, YPR036W
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3221
Unit cell:
a: 117.342Å b: 117.342Å c: 119.873Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.312
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

Vacuolar ATPases: rotary proton pumps in physiology and pathophysiology.
Forgac M. (2007)
25 more

21 mentions without citation

Breaking up and making up: The secret life of the vacuolar H+ -ATPase.
Oot et al. (2017)
20 more