PDBe 1hjs

X-ray diffraction
1.87Å resolution

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.

Released:

Function and Biology Details

Reaction catalysed:
The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans. 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arabinogalactan endo-beta-1,4-galactanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 332 amino acids
Theoretical weight: 36.84 KDa
Source organism: Thermothelomyces thermophila
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P83692 (Residues: 1-332; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 53
Structure domains: Glycosidases

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2
Unit cell:
a: 108.375Å b: 136.145Å c: 111.007Å
α: 90° β: 119.02° γ: 90°
R-values:
R R work R free
0.194 0.194 0.208
Expression system: Aspergillus oryzae