PDBe 1hf0

X-ray diffraction
2.7Å resolution

Crystal structure of the DNA-binding domain of Oct-1 bound to DNA as a dimer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
POU domain, class 2, transcription factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14859 (Residues: 280-438; Coverage: 21%)
Gene names: OCT1, OTF1, POU2F1
Sequence domains:
Structure domains:
DNA 5'-D(*CP*AP*CP*AP*TP*TP*TP*GP*AP*AP*AP*GP*GP* CP*AP*AP*AP*TP*GP*GP*AP*G)-3' Chain: M
Molecule details ›
Chain: M
Length: 22 nucleotides
Theoretical weight: 6.83 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA 5'-D(*CP*TP*CP*CP*AP*TP*TP*TP*GP*CP*CP*TP*TP* TP*CP*AP*AP*AP*TP*GP*TP*G)-3' Chain: N
Molecule details ›
Chain: N
Length: 22 nucleotides
Theoretical weight: 6.67 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P6222
Unit cell:
a: 131.25Å b: 131.25Å c: 116.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.239 0.294
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided