PDBe 1hdd

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF AN ENGRAILED HOMEODOMAIN-DNA COMPLEX AT 2.8 ANGSTROMS RESOLUTION: A FRAMEWORK FOR UNDERSTANDING HOMEODOMAIN-DNA INTERACTIONS

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Segmentation polarity homeobox protein engrailed Chains: C, D
Molecule details ›
Chains: C, D
Length: 61 amino acids
Theoretical weight: 7.47 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P02836 (Residues: 453-512; Coverage: 11%)
Gene names: CG9015, en
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
DNA (5'-D(*AP*TP*TP*AP*GP*GP*TP*AP*AP*TP*TP*AP*CP*AP*TP*GP*G P*CP*AP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*TP*GP*CP*CP*AP*TP*GP*TP*AP*AP*TP*TP*AP*C P*CP*TP*AP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 131.2Å b: 45.5Å c: 72.9Å
α: 90° β: 119° γ: 90°
R-values:
R R work R free
0.225 0.225 not available
Expression systems:
  • Escherichia coli
  • Not provided