› 1ha5

X-ray diffraction
2.82Å resolution

Structural features of a zinc-binding site in the superantigen streptococcal pyrogenic exotoxin A (SpeA1): implications for MHC class II recognition.

Released:
DOI: 10.2210/pdb1ha5/pdb
PDB entry 1ha5 coloured by chain, front view.
PDB entry 1ha5 coloured by chain, side view.
PDB entry 1ha5 coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Structural features of a zinc binding site in the superantigen strepococcal pyrogenic exotoxin A (SpeA1): implications for MHC class II recognition.
Baker M, Gutman DM, Papageorgiou AC, Collins CM, Acharya KR
Protein Sci 10 1268-73 (2001)
PMID: 11369867

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exotoxin type A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 218 amino acids
Theoretical weight: 25.38 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DJY7 (Residues: 34-169, 170-249; Coverage: 98%)
Gene name: speA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21212
Unit cell:
a: 126.946Å b: 101.32Å c: 82.019Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.283
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Disease manifestations and pathogenic mechanisms of Group A Streptococcus.
Walker et al. (2014)
5 more

1 mention without citation

Structural features of a zinc binding site in the superantigen strepococcal pyrogenic exotoxin A (SpeA1): implications for MHC class II recognition.
Baker et al. (2001)