PDBe 1h9o

X-ray diffraction
1.79Å resolution

PHOSPHATIDYLINOSITOL 3-KINASE, P85-ALPHA SUBUNIT: C-TERMINAL SH2 DOMAIN COMPLEXED WITH A TYR751 PHOSPHOPEPTIDE FROM THE PDGF RECEPTOR, CRYSTAL STRUCTURE AT 1.79 A

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biological process:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 3-kinase regulatory subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27986 (Residues: 616-724; Coverage: 15%)
Gene names: GRB1, PIK3R1
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein
Platelet-derived growth factor receptor beta Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 702 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P09619 (Residues: 751-755; Coverage: 1%)
Gene names: PDGFR, PDGFR1, PDGFRB

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: C2
Unit cell:
a: 59Å b: 32.8Å c: 54.9Å
α: 90° β: 96.2° γ: 90°
R-values:
R R work R free
0.168 0.163 0.219
Expression systems:
  • Escherichia coli
  • Not provided