PDBe 1h89

X-ray diffraction
2.45Å resolution

CRYSTAL STRUCTURE OF TERNARY PROTEIN-DNA COMPLEX2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcriptional activator Myb Chain: C
Molecule details ›
Chain: C
Length: 159 amino acids
Theoretical weight: 19.17 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06876 (Residues: 35-193; Coverage: 25%)
Gene name: Myb
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
CCAAT/enhancer-binding protein beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 64 amino acids
Theoretical weight: 7.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17676 (Residues: 273-336; Coverage: 19%)
Gene names: CEBPB, PP9092, TCF5
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
DNA(5'-(*CP*CP*AP*GP*TP*CP*CP*GP*TP*TP*AP* AP*GP*GP*AP*TP*TP*GP*CP*GP*CP*CP*AP*CP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 26 nucleotides
Theoretical weight: 7.95 KDa
DNA(5'-(*GP*AP*TP*GP*TP*GP*GP*CP*GP*CP*AP* AP*TP*CP*CP*TP*TP*AP*AP*CP*GP*GP*AP*CP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 26 nucleotides
Theoretical weight: 8.03 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P212121
Unit cell:
a: 62.684Å b: 73.383Å c: 161.699Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.267
Expression system: Escherichia coli BL21(DE3)