PDBe 1h7k

X-ray diffraction
2.4Å resolution

Formation of a tyrosyl radical intermediate in Proteus mirabilis catalase by directed mutagenesis and consequences for nucleotide reactivity

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chain: A
Molecule details ›
Chain: A
Length: 483 amino acids
Theoretical weight: 55.61 KDa
Source organism: Proteus mirabilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42321 (Residues: 2-484; Coverage: 100%)
Gene name: katA
Sequence domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6222
Unit cell:
a: 110Å b: 110Å c: 251.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.24
Expression system: Escherichia coli