PDBe 1h4g

X-ray diffraction
1.1Å resolution

OLIGOSACCHARIDE-BINDING TO FAMILY 11 XYLANASES: BOTH COVALENT INTERMEDIATE AND MUTANT-PRODUCT COMPLEXES DISPLAY 2,5B CONFORMATIONS AT THE ACTIVE-CENTRE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 23.16 KDa
Source organism: [Bacillus] agaradhaerens
Expression system: Bacillus licheniformis
UniProt:
  • Canonical: Q7SIE3 (Residues: 1-207; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 72.061Å b: 75.098Å c: 78.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.181
Expression system: Bacillus licheniformis