PDBe 1h3e

X-ray diffraction
2.9Å resolution

Tyrosyl-tRNA synthetase from Thermus thermophilus complexed with wild-type tRNAtyr(GUA) and with ATP and tyrosinol


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Tyrosine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 48.79 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P83453 (Residues: 1-432; Coverage: 100%)
Gene names: TT_C1033, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:
Molecule details ›
Chain: B
Length: 86 nucleotides
Theoretical weight: 27.71 KDa
Source organism: Thermus thermophilus
Expression system: Not provided

Ligands and Environments

2 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3121
Unit cell:
a: 129.525Å b: 129.525Å c: 109.451Å
α: 90° β: 90° γ: 120°
R R work R free
0.219 0.219 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided