PDBe 1h27

X-ray diffraction
2.2Å resolution

CDK2/CyclinA in complex with an 11-residue recruitment peptide from p27

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-A2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 259 amino acids
Theoretical weight: 29.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: P20248 (Residues: 174-432)
Gene names: CCN1, CCNA, CCNA2
Cyclin-dependent kinase 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 303 amino acids
Theoretical weight: 34.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: P24941 (Residues: 1-298)
Gene names: CDK2, CDKN2
Cyclin-dependent kinase inhibitor 1B Chain: E
Molecule details ›
Chain: E
Length: 11 amino acids
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Best match: P46527 (Residues: 25-35)
Gene names: CDKN1B, KIP1

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 73.687Å b: 133.547Å c: 148.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.269
Expression systems:
  • Escherichia coli
  • Not provided