PDBe 1h24

X-ray diffraction
2.5Å resolution

CDK2/CYCLIN A IN COMPLEX WITH A 9 RESIDUE RECRUITMENT PEPTIDE FROM E2F

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-A2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 259 amino acids
Theoretical weight: 29.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20248 (Residues: 174-432; Coverage: 60%)
Gene names: CCN1, CCNA, CCNA2
Sequence domains:
Structure domains: Cyclin A; domain 1
Transcription factor E2F1 Chain: E
Molecule details ›
Chain: E
Length: 9 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q01094 (Residues: 87-95; Coverage: 2%)
Gene names: E2F1, RBBP3
Cyclin-dependent kinase 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 303 amino acids
Theoretical weight: 34.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P24941 (Residues: 1-298; Coverage: 100%)
Gene names: CDK2, CDKN2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 73.551Å b: 133.549Å c: 147.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.27
Expression systems:
  • Escherichia coli
  • Not provided