› 1gyc

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE DETERMINATION AT ROOM TEMPERATURE OF A LACCASE FROM TRAMETES VERSICOLOR IN ITS OXIDISED FORM CONTAINING A FULL COMPLEMENT OF COPPER IONS

Released:
DOI: 10.2210/pdb1gyc/pdb
PDB entry 1gyc coloured by chain, front view.
PDB entry 1gyc coloured by chain, side view.
PDB entry 1gyc coloured by chain, top view.
Source organism: Trametes versicolor
Primary publication:
Crystal structure of a laccase from the fungus Trametes versicolor at 1.90-A resolution containing a full complement of coppers.
Piontek K, Antorini M, Choinowski T
J Biol Chem 277 37663-9 (2002)
PMID: 12163489

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Laccase-2 Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 53.68 KDa
Source organism: Trametes versicolor
UniProt:
  • Canonical: Q12718 (Residues: 21-519; Coverage: 100%)
Gene names: LCC2, LCCI
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand CU 4 x CU
Ligand NAG 3 x NAG
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 83.681Å b: 84.977Å c: 91.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.212

Quick links

Citations

40 review citations

Fungal laccases - occurrence and properties.
Baldrian P. (2006)
39 more

55 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
54 more