PDBe 1gxc

X-ray diffraction
2.7Å resolution

FHA domain from human Chk2 kinase in complex with a synthetic phosphopeptide

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SYNTHETIC PHOSPHOPEPTIDE Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 10 amino acids
Theoretical weight: 1.46 KDa
Source organism: synthetic construct
Expression system: Not provided
Serine/threonine-protein kinase Chk2 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 149 amino acids
Theoretical weight: 17.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O96017 (Residues: 64-212; Coverage: 27%)
Gene names: CDS1, CHEK2, CHK2, RAD53
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 79.824Å b: 82.884Å c: 129.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.249
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)