PDBe 1gu4

X-ray diffraction
1.8Å resolution

Crystal structure of C/EBPBETA BZIP homodimer bound to a high affinity DNA fragment

Released:
Entry authors: Tahirov TH, Ogata K

Function and Biology Details

Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CCAAT/enhancer-binding protein beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 78 amino acids
Theoretical weight: 9.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17676 (Residues: 259-336; Coverage: 23%)
Gene names: CEBPB, PP9092, TCF5
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
5'-D(*AP*AP*TP*AP*TP*TP*GP*CP*GP*CP* AP*AP*TP*CP*CP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*TP*AP*GP*GP*AP*TP*TP*GP*CP*GP* CP*AP*AP*TP*AP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: C2221
Unit cell:
a: 100.947Å b: 112.326Å c: 74.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.266
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided