PDBe 1grv

X-ray diffraction
2.9Å resolution

Hypoxanthine Phosphoribosyltransferase from E. coli

Released:

Function and Biology Details

Reaction catalysed:
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9M2 (Residues: 1-178; Coverage: 100%)
Gene names: JW5009, b0125, hpt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P3121
Unit cell:
a: 83.9Å b: 83.9Å c: 169.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.238
Expression system: Escherichia coli